M-phase promoting factor (MPF) and mitogen activated protein kinases (MAPK) activities of domestic cat oocytes matured <i>in vitro</i> and <i>in vivo</i>

This work was undertaken in order to examine M-phase promoting factor (MPF) and mitogen-activated protein kinases (MAPK) activities during meiotic progression of cat oocytes cultured in two different media for two different incubation times and preovulatory cat oocytes that reached MII in vivo. Oocytes recovered from ovaries of ovariectomized cats were cultured either in TCM 199 or SOF for 24 h and 40 h. In vivo matured oocytes were recovered by follicular aspiration from ovaries of domestic cats ovariectomized 24 h to 26 h after hormonal treatment. Results showed that the kinetic of MPF and MAPK activity was similar during meiotic progression of cat oocytes matured in TCM 199 and SOF. After 24 h of incubation, MII oocytes had significantly (p &lt; 0.001) higher MPF and MAPK levels than MII oocytes cultured for 40 h in both culture media. MPF and MAPK activity was significantly (p &lt; 0.01) lower in the oocytes matured in vitro than in those matured in vivo. This study provides evidence that the two different maturation media did not determine differences in MPF and MAPK fluctuations and levels during meiotic progression of cat oocytes and that the time of maturation influenced the level of the two kinases. Moreover, it shows that MPF and MPK activity is higher in in vivo matured oocytes than in in vitro matured oocytes, suggesting a possible incomplete cytoplasmic maturation after culture

Reduction of burden of hemolyzed specimens in a large urban emergency department: A real-world, five years’ experience

In vitro hemolysis may jeopardize patient care because tests results generated using unsuitable specimens may lead to inappropriate patient management. The prevalence of hemolyzed specimens is high in the emergency department (ED). We previously showed that collecting blood by means of a closed system entailing manual aspiration of blood instead of using conventional evacuated systems was effective to cut-down by nearly half the rate of hemolysis. Aim of this real world study was to verify whether longterm replacement of standard evacuated blood collection systems may be really effective to reduce the burden of spurious hemolysis. Starting from May 2014 in the ED of our Hospital vacuum tubes were replaced with S-Monovette serum tubes. We compared data about hemolyzed specimens entered in the two years before the implementation of the new device (i.e., 2012 and 2013) and the two years after introducing SMonovette in manual aspiration mode (i.e. 2015 and 2016). The year 2014 was not considered due to mixed data. The rate of hemolyzed specimens decreased from 4.36% to 3.07% with the use of S-Monovette in manual aspiration mode (Chi squared, 183.8; P&lt;0.001). The likelihood of obtaining hemolyzed specimens was hence reduced by approximately 30% (relative risk, 0.707), with an expected economic saving of approximately 510€/year. The results of this real-world study demonstrate that the use of an alternative closed device encompassing manual aspiration for drawing blood from intravenous catheters may reduce hemolyzed samples by approximately 30%, so representing a valuable perspective for safeguarding patient safety and improving ED efficiency

Magnetic Resonance Imaging of Optic Nerve Traction During Adduction in Primary Open-Angle Glaucoma With Normal Intraocular Pressure.

PurposeWe used magnetic resonance imaging (MRI) to ascertain effects of optic nerve (ON) traction in adduction, a phenomenon proposed as neuropathic in primary open-angle glaucoma (POAG).MethodsSeventeen patients with POAG and maximal IOP ≤ 20 mm Hg, and 31 controls underwent MRI in central gaze and 20° to 30° abduction and adduction. Optic nerve and sheath area centroids permitted computation of midorbital lengths versus minimum paths.ResultsAverage mean deviation (±SEM) was -8.2 ± 1.2 dB in the 15 patients with POAG having interpretable perimetry. In central gaze, ON path length in POAG was significantly more redundant (104.5 ± 0.4% of geometric minimum) than in controls (102.9 ± 0.4%, P = 2.96 × 10-4). In both groups the ON became significantly straighter in adduction (28.6 ± 0.8° in POAG, 26.8 ± 1.1° in controls) than central gaze and abduction. In adduction, the ON in POAG straightened to 102.0% ± 0.2% of minimum path length versus 104.5% ± 0.4% in central gaze (P = 5.7 × 10-7), compared with controls who straightened to 101.6% ± 0.1% from 102.9% ± 0.3% in central gaze (P = 8.7 × 10-6); and globes retracted 0.73 ± 0.09 mm in POAG, but only 0.07 ± 0.08 mm in controls (P = 8.8 × 10-7). Both effects were confirmed in age-matched controls, and remained significant after correction for significant effects of age and axial globe length (P = 0.005).ConclusionsAlthough tethering and elongation of ON and sheath are normal in adduction, adduction is associated with abnormally great globe retraction in POAG without elevated IOP. Traction in adduction may cause mechanical overloading of the ON head and peripapillary sclera, thus contributing to or resulting from the optic neuropathy of glaucoma independent of IOP

La formación docente inicial concepciones y prácticas curriculares

El trabajo se centra en el currículo de Formación Docente de Educación Superior para los Niveles Inicial y Primario de la Dirección General y Cultura de la Provincia de Buenos Aires, ideado, coordinado e impulsado por Jorge Huergo. Tiene el propósito de recuperar su pensamiento y acción en la enseñanza formal, consecuente con su perspectiva crítica latinoamericana. Se toma en especial consideración uno de sus sentidos nucleares: la centralidad de las prácticas de formación en la dimensión sociocultural amplia y, en esa línea, su reinserción en los ámbitos e instituciones sociocomunitarios. Esta innovación –el currículo y sus prácticas– guarda extraordinaria coherencia en sus componentes y procesos desarrollados. Se comentan algunos conceptos troncales y estrategias, tanto de diseño como de desarrollo curricular. Por otra parte, se consignan comentarios de su impacto institucional a partir de manifestaciones de algunos docentes de institutos que participaron y sostienen la experiencia.

Graphic arts in the National Book Exhibition of 1928: Materiality, art and industry in book production in Argentina

En septiembre de 1928 tuvo lugar en Buenos Aires la Primera Exposición Nacional del Libro, evento que aglutinó gran parte de las discusiones en torno al desarrollo de la industria editorial en la década de 1920, al tiempo que representó un hito significativo para la consolidación de las artes gráficas en Argentina.En las primeras décadas del siglo XX diversas iniciativas institucionales configuraron, paulatinamente, una tradición del grabado como un arte del libro, aliado de la ilustración literaria. La Exposición colaboró en gran medida con este proceso: destinó un espacio dedicado a la exhibición de grabados y ediciones de lujo y contó con una comisión especial de ilustradores integrada, entre otros, por Adolfo Bellocq y Alfredo Guido quienes desplegaron una intensa labor como promotores del grabado de ilustración desde la práctica artística y pedagógica. Asimismo, se recurrió a una estrategia para promocionar el evento que contaba con cierta tradición en el mundo de la gráfica: el concurso de carteles artísticos.Así, se analiza esta exposición en sus aspectos vinculados con la circulación de imágenes e ideas en torno al libro como objeto y, particularmente, las tempranas tensiones referidas a las artes gráficas en el contexto de la industria editorial argentina.In September 1928 was held in Buenos Aires the First National Book Exhibition, an event that agglutinated much of the discussions around the advancement of the publishing industry in the 1920’s, while representing a significant milestone for the consolidation of graphic arts in Argentina. In the first decades of the XXth century, diverse institutional initiatives gradually shaped a tradition of engraving as a “book art”, allied to literary illustration. e Exhibition collaborated to a large extent with this process: it assigned a space for an engraving and luxury editions exhibition, and it counted with the attendance of a special commission of illustrators, formed by Adolfo Bellocq and Alfredo Guido, among others, who worked intensely as promoters of engraving trade from both artistic and pedagogical practice. Furthermore, the strategy that was used to promote the event had a certain tradition in graphic arts: the artistic poster contest. We propose to analyze this exhibition in its aspects related to the circulation of images and ideas around the book like an object as its own right and, particularly, the early tensions related to graphic arts within the Argentine publishing industry.Fil: Bonelli Zapata, Ana Laura. Universidad Nacional de San Martin. Centro de Investigaciones En Arte y Patrimonio. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Pque. Centenario. Centro de Investigaciones En Arte y Patrimonio.; ArgentinaFil: Villanueva, Aldana. Universidad Nacional de San Martin. Centro de Investigaciones En Arte y Patrimonio. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Pque. Centenario. Centro de Investigaciones En Arte y Patrimonio.; Argentin

Sorption-enhanced steam reforming of ethanol for hydrogen production

In last years, sorption-enhanced steam reforming of ethanol (SESRE) has become an interesting alternative for the industry, since it offers high H2 purity with a simpler and smaller system. In this study CaO derived materials were synthesized to improve multicyclic CO2 sorption-desorption stability. A dispersant and inert phase (Ca12All4O33) was incorporated into CaO structure. The effects of CaO:Ca12Al14O33 ratio on the characteristics of the new absorbents and on their performance in sorption-enhanced steam reforming of ethanol were analyzed. The results obtained indicate that the absorbent 85% CaO – 15% Ca12Al14O33 had significantly improved cyclic reaction stability. Additionally, it was proved that with this adsorbent CO purity on wet basis was lower than 2 %, making it possible to attain in only one reaction stage the CO purity obtained after de WGS reactor.Fil: Avendaño Cañizalez, Roger Orlando. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Tecnologías del Hidrogeno y Energias Sostenibles. Universidad de Buenos Aires. Facultad de Ingeniería. Instituto de Tecnologías del Hidrogeno y Energias Sostenibles; ArgentinaFil: Dieuzeide, María Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Tecnologías del Hidrogeno y Energias Sostenibles. Universidad de Buenos Aires. Facultad de Ingeniería. Instituto de Tecnologías del Hidrogeno y Energias Sostenibles; ArgentinaFil: Bonelli, Pablo Ricardo. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Amadeo, Norma Elvira. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Tecnologías del Hidrogeno y Energias Sostenibles. Universidad de Buenos Aires. Facultad de Ingeniería. Instituto de Tecnologías del Hidrogeno y Energias Sostenibles; Argentin

The use of S-Monovette is effective to reduce the burden of hemolysis in a large urban emergency department

Background: Due to the high prevalence of hemolysis in specimens received from the emergency department (ED), several strategies have been proposed to improve sample quality, but none of these seem effective to overcome the problem. In a preliminary study we showed that the use of S-Monovette blood collection system was effective to lower the risk of hemolysis in venous blood samples collected from intravenous catheters. This study was hence aimed to verify whether the replacement of a conventional vacuum system with S-Monovette may be effective to reduce the burden of hemolysis in the daily practice of a large urban ED. Materials and methods: The study was divided in two observational periods of 4 months each. In the former period, blood was collected from intravenous catheters using BD Vacutainer SST II Plus plastic serum tubes, whereas in the latter period the blood was drawn from intravenous catheters using S-Monovette blood tubes in aspiration mode. Sample hemolysis was automatically assessed in all serum samples by photometrical measurement. Results: The total number of hemolysed serum specimens was 624/14155 (4.41%) in the first phase of the study, and 342/13319 (2.57%) in the second phase of the study (P < 0.001). Conclusion: Results of our study confirm that the introduction of the Sarstedt S-Monovette blood tubes has reduced the hemolysis rate in the emergency department compared to the previously used BD Vacutainer® SST II Plus plastic serum tube

Una mirada a la identidad de la extensión universitaria en la UNLP

La identidad de la extensión universitaria refiere a la percepción y autopercepción institucional que se construye y se sostiene estructural y relacionalmente a lo largo del tiempo, en término de las funciones que le son conferidas. Se configura desde las raíces identitarias de origen de la universidad y en continua interacción entre factores internos y externos. Los proyectos de extensión, como “seña” de identidad institucional, expresan el lugar de la institución, del otro, del conocimiento / saber, y de la relación que se construye. A los efectos de este trabajo, estas categorías de análisis, se consideran a los fines de rastrear las visiones y concepciones que subyacen como ideario en las propuestas de extensión universitaria y que contribuyen a la configuración del proyecto institucional. La identidad entendida como proceso complejo en el que intervienen aspectos subjetivos, psicosociales y culturales, asociado a condiciones sociohistóricas y estructurales, remite a ser abordada tal como está expresada en el proyecto institucional; a la definición de lo que ha sido, y a lo que va siendo, según puede explorarse en las recurrencias evidenciadas en las propuestas extensionista.Facultad de Humanidades y Ciencias de la Educació

Reduction of burden of hemolyzed specimens in a large urban emergency department: A real-world, five years' experience

In vitro hemolysis may jeopardize patient care because tests results generated using unsuitable specimens may lead to inappropriate patient management. The prevalence of hemolyzed specimens is high in the emergency department (ED). We previously showed that collecting blood by means of a closed system entailing manual aspiration of blood instead of using conventional evacuated systems was effective to cut-down by nearly half the rate of hemolysis. Aim of this real world study was to verify whether longterm replacement of standard evacuated blood collection systems may be really effective to reduce the burden of spurious hemolysis. Starting from May 2014 in the ED of our Hospital vacuum tubes were replaced with S-Monovette serum tubes. We compared data about hemolyzed specimens entered in the two years before the implementation of the new device (i.e., 2012 and 2013) and the two years after introducing SMonovette in manual aspiration mode (i.e. 2015 and 2016). The year 2014 was not considered due to mixed data. The rate of hemolyzed specimens decreased from 4.36% to 3.07% with the use of S-Monovette in manual aspiration mode (Chi squared, 183.8; P<0.001). The likelihood of obtaining hemolyzed specimens was hence reduced by approximately 30% (relative risk, 0.707), with an expected economic saving of approximately 510€/year. The results of this real-world study demonstrate that the use of an alternative closed device encompassing manual aspiration for drawing blood from intravenous catheters may reduce hemolyzed samples by approximately 30%, so representing a valuable perspective for safeguarding patient safety and improving ED efficiency

Civility and Trust in Social Media

Abstract Social media have been credited with the potential of reinvigorating trust by offering new opportunities for social and political participation. This view has been recently challenged by the rising phenomenon of online incivility, which has made the environment of social networking sites hostile to many users. We conduct a novel experiment in a Facebook setting to study how the effect of social media on trust varies depending on the civility or incivility of online interaction. We find that participants exposed to civil Facebook interaction are significantly more trusting. In contrast, when the use of Facebook is accompanied by the experience of online incivility, no significant changes occur in users' behavior. These results are robust to alternative configurations of the treatments